A cloned buffalo, claimed to be the first-of-its-kind bovine in the world, 
on Friday delivered a calf at National Dairy Research Institute (NDRI), Karnal. The newborn female calf was named 'Mahima'.
In 2009, the researchers at NDRI reportedly produced the world's first cloned buffalo through 'advanced hand-guided cloning technique'. But the clone could survive for just six days. Even the second clone had died in 2011 due to aliments after staying alive for two years.
But Garmia II, which was the third cloned buffalo born on August 22, 2010, not only survived without any complications but delivered a calf at 1.51am on Friday.
Director of the institute A K Srivastava said 'Mahima', weighing 32kg, was born by normal parturition. He said on Friday morning, the clone showed impending calving symptoms and was given medical care.
He said the calf was keeping very good health and started sucking milk within 30 minutes of its birth. The director said that the pregnancy was maintained under standard scientific management system during the gestation period. The director said the institute was continuing further research technique for producing more cloned animals.
Hand-guided cloning technique is an advanced modification of the conventional cloning technique that requires expensive micromanipulator machines. In this technique, immature oocytes are isolated from ovaries and are matured in-vitro. These are then denuded, treated with an enzyme to digest the outer covering called 'zona pellucida' and then treated with chemicals to push their genetic material to one side. This side is then cut off with the help of a hand-held fine microblade to remove the genetic material. The enucleated oocyte is then electrofused with a somatic cell taken from any source. The resulting embryos are cultured and grown in the laboratory for about seven days to develop them to the blastocyst stage. The embryos are then transferred to recipient buffaloes for producing cloned offspring.
on Friday delivered a calf at National Dairy Research Institute (NDRI), Karnal. The newborn female calf was named 'Mahima'.
In 2009, the researchers at NDRI reportedly produced the world's first cloned buffalo through 'advanced hand-guided cloning technique'. But the clone could survive for just six days. Even the second clone had died in 2011 due to aliments after staying alive for two years.
But Garmia II, which was the third cloned buffalo born on August 22, 2010, not only survived without any complications but delivered a calf at 1.51am on Friday.
Director of the institute A K Srivastava said 'Mahima', weighing 32kg, was born by normal parturition. He said on Friday morning, the clone showed impending calving symptoms and was given medical care.
He said the calf was keeping very good health and started sucking milk within 30 minutes of its birth. The director said that the pregnancy was maintained under standard scientific management system during the gestation period. The director said the institute was continuing further research technique for producing more cloned animals.
Hand-guided cloning technique is an advanced modification of the conventional cloning technique that requires expensive micromanipulator machines. In this technique, immature oocytes are isolated from ovaries and are matured in-vitro. These are then denuded, treated with an enzyme to digest the outer covering called 'zona pellucida' and then treated with chemicals to push their genetic material to one side. This side is then cut off with the help of a hand-held fine microblade to remove the genetic material. The enucleated oocyte is then electrofused with a somatic cell taken from any source. The resulting embryos are cultured and grown in the laboratory for about seven days to develop them to the blastocyst stage. The embryos are then transferred to recipient buffaloes for producing cloned offspring.
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